Real time PCR

Instrument Details

Make : Applied Biosystems

Model :ABI StepOneTM

Specification :

The StepOne™ Real-Time PCR System is a 48-well, low-throughput robust LED based 3-color optical recording instrument for genomic research. It offer intuitive software, Fast (<40 min) and standard (<2 hr) runs, and are configured for PC-free or networked operation.
Simple and flexible instrument set-up and usage.

The instrument illuminates all wells of the reaction plate, exciting the fluorophores in each reaction, and collects the residual fluorescence emitted from the wells of the reaction plate.
The resulting image collected by the device consists only of light that corresponds to the range of emission wavelengths and further assembles a digital representation of the residual fluorescence collected over a fixed time interval.

MAIN FEATURES OF THE INSTRUMENT

Applications

• SNP genotyping, gene expression analysis
• smicroRNA expression, translocation analysis, gene detection
• viral load analysis
• quantitative detection of target nucleic acid sequences (targets) using real-time analysis
• qualitative detection of targets using post-PCR (endpoint) analysis
• qualitative analysis of the PCR

References

User Instructions

1. Users are advised to send high quality cDNA and DNA along with suitable primers (minimum 48 well)

Contact Us

Email Id : chandramohan.sangh@icar.gov.in

Real time PCR charges (Rs)includes GST

	Industry	University	National Lab/R&D's
	1000	500	800	Cost Per Sample