DGR

Ion Chromatograph

Instrument Details

Make : Dionex Ion Chromatography System, USA

Model : ICS 3000

Specification :

IC containing Suction Pump (SP), Conductivity Detector (CD), Electrochemical Detector (ED) and Variable Wavelength Detector (VWD)

Ion chromatography separates ions and polar molecules based on their affinity to the ion exchanger and works on any kind of charged molecule including large proteins, small nucleotides, and amino acids.
Anion and cation-exchange are two types of ion chromatography. The water-soluble and charged molecules bind to moieties which are oppositely charged by forming ionic bonds to the insoluble stationary phase.
The equilibrated stationary phase consists of an ionizable functional group where the targeted molecules of a mixture to be separated and quantified can bind while passing through the column.
A cationic stationary phase is used to separate anions and an anionic stationary phase is used to separate cations.

MAIN FEATURES OF THE INSTRUMENT

Feature Isocratic Pump Gradient Pump

Pump Dual-piston (in series), variable speed, 80 μL per revolution Dual-piston (in series), variable speed, 80 μL per revolution

Flow Rate Range 0.001 to 10.0 mL/min in 0.001 mL increments 0.001 to 10.0 mL/min in increments of 0.001 mL/min

Flow Rate Accuracy and Reproducibility ±0.1% at 1.0 mL/min (typical) ±0.1% at 1.0 mL/min (typical)

Operating Pressure 0.1 to 35 MPa (15 to 5000 psi) 0.1 to 35 MPa (15 to 5000 psi)

Pressure Ripple 1% (typical) 1% (typical)

Gradient Accuracy and Reproducibility Not applicable ±0.5% at 2.0 mL/min (typical)

Number of Eluents One Four

Gradient Mixer Delay Volume Not applicable GM-3: <500 μL, 3- 9 mm ID columns

High Pressure Limit 0 to 35 MPa (0 to 5000 psi) in 0.1 increments 0 to 35 MPa (0 to 5000 psi) in 0.1 increment

Low Pressure Limit 0 to 34 MPa (0 to 4900 psi) in 0.1 increments 0 to 34 MPa (0 to 4900 psi) in 0.1 increment

Vacuum Degassing Module Built-in single-channel degas chamber with internal volume of 670 µL and dual-stage diaphragm vacuum pump Built-in four-channel degas chamber with internal volume of 670 μL per channel and dual-stage diaphragm vacuum pump

Conductivity Detector (CD)

Electronics Microprocessor-controlled digital signal processing

Detection Types Single- or dual-conductivity detection. Dual-detection controlled either simultaneously or independently

Cell Drive 8 kHz square wave

Linearity 1% at 1000 µS, Resolution 0.10 nS

Signal Sampling Rate Maximum of 100 Hz

Electronic Noise Dry S/N ratio = 200,000:1 (Example: 1000 µS:5 nS), Wet: At 23 µS, noise <0.2 nS, At 1 µS, noise <0.1 nS

Filter Rise times from 0 to 10 s

Cell Body PEEK

Cell Electrodes Passivated 316 stainless steel

Active Volume of Cell < 1.0 µL

Cell Operating Temperature 5 ºC above the DC detector compartment temperature; maximum of 60 ºC

Cell Maximum Operating Pressure 3 MPa (500 psi)

Signal Gain Ranges 0 to 180 µS, 0 to 1000 µS, and 0 to 15,000 µS

Optional Analog Output Ranges Three separate ranges from 0 to 15,000 µS

Electrochemical Detector (ED)

Electronics Microprocessor-controlled digital signal processing

Detection Types Single- or dual-electrochemical detection. Dual-detection can be controlled either simultaneously or independently. Noise < 1 pA (DC Amperometry) 10 pC (Integrated Amperometry)

Cell Drive Potential Settings 2.00 to 2.00 V in 0.001 V increments

Optional Analog Output Range 50 pC to 200 μC (Integrated Amperometry) 5 pA to 74 μA (DC Amperometry)

Cell Working Electrode Disposable gold, silver, or platinum, Non-disposable platinum, gold, silver, or glassy carbon

Cell Counter Electrode Titanium

Cell Reference Electrode pH-Ag/AgCl combination

Wetted Materials PEEK, titanium, Kel-F, polyetherimide, EPR, glass, high density polyethylene (HDPE), ULTEM, Teflon working electrode materials

Cell Volume at Working Electrode < 0.5 µL ; Cell Maximum Operating Pressure 0.7 MPa (100 psi)

Variable Wavelength Detector (VWD)

Type Variable Wavelength Detector (Single Channel)

Data Collection Rate Up to 10Hz,

Analog Outputs Two: absorbance, 20-bit resolution, 0-1V and 0-10V , Drift <0.1mAU/hr, Noise <±2.5 μAU at 254nm

Wavelength Range 190 to 900nm, Accuracy ±1nm, Tungsten and Deuterium Lamps simultaneous

Flow Celle PEEK, 11µL volume, 10mm path length (analytical); 2.5µL, 7mm path (semimicro), up to 2.5AU Linearity

Wavelength Bandwidth 6 nm at 254 nm; Hertz 47/63Hz

Applications

• Anion, cation and organic acid analysis from soil and plants.
• Analysis of Individual sugars from seed, leaf, stems and root samples.
• Phenols profiling from seed, leaf, stem and root samples.

References

1. https://tools.thermofisher.com/content/sfs/manuals/48428-Man-IC-ICS-3000-System-Operators-2008-01-065031-04.pdf
2. http://www.dionex-france.com/library/manuals/IC/detectors/PIU_ED-001.pdf
3. SK Bishi, L Kumar, MK Mahatma, N.Khatediya, SM Chauhan, JB Misra, (2015) Quality traits of Indian peanut cultivars and their utility as nutritionaland functional food. Food Chemistry. 167, 107-114.
4. Bariya, H. A.L. Singh and Vidya Chaudhari 2015. Measurement of Fe(II) and Fe(III) in Groundnut by In-column and Post Column Reactions in Ion-chromatography. Communications in Soil Science and Plant Analysis, 46:358–366.

User Instructions

1. Sample should be submitted in solution form specifying the element to be estimated.
2. Details of sample type, exact weight and dilution need to be mentioned for integration.
3. Generally 5-10 ml of solution is sufficient for estimation.
4. For special sample, appropriate standards should be submitted by the user.

Contact : +91-9426220996
Email Id : amrit.singh@icar.gov.in

Ion Chromatograph charges (Rs)includes GST

	Industry	University	National Lab/R&D's
Standardization	3500	1000	2000	Per parameter
Subsequent Sample estimation	1000	400	600	Per Sample
Sample preparation	7000	1500	3000	Per Sample